eng
National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)
Egyptian Journal of Veterinary Sciences
1110-0222
2357-089X
2016-12-01
47
2
111
115
10.21608/ejvs.2016.1069
1069
Unexpected Presence of Diatom in Pathological Specimens of Fetal Membrane of Aborted Cattle Case Report
Youssef Ahmed
yfahmed54@yahoo.com
1
Prof.emeritus
THE OBJECTIVE of this article was to report diatom in aborted …… fetal membrane of cattle. Tissue samples of aborted fetal membrane were fixed in 10% neutral buffered formalin and submitted for pathological evaluation. The tissue samples were prepared for examination and stained with Hematoxylin and eosin, Gram, Gomori’s methenamine-silver and Periodic Acid Schiff, and examined using research and phase - contrast microscope. Histopathological examination showed different shapes of diatoms. The most common type of diatoms in the examined tissues was reported. In conclusions, this report is considered the first detection of diatoms in a tissue of aborted fetal membrane of a dairy cows. The source of infection may be from drinking contaminated water, however no pathological changes could be detected around diatoms and the relationship between abortion and algae infection was not detected and further studies were carried out regarding this point.
https://ejvs.journals.ekb.eg/article_1069_944038e8ba87dc79da4004e472caf08e.pdf
diatom
Abortion
cattle
Pathological changes
eng
National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)
Egyptian Journal of Veterinary Sciences
1110-0222
2357-089X
2016-12-01
47
2
165
177
10.21608/ejvs.2017.3591
3591
Original Article
Efficacy of Some Anticoccidial Drugs on Experimentally Induced Cecal Coccidiosis (E. tsunodai) in Japanese Quails
OOCYSTS of different Eimeria species were isolated from 27 farms of Japanese quail which collected from Al-Dakahlia and Kafr El-Sheikh provinces, Egypt. Eimeria isolates were identified as E. bateri, E. uzura and E. tsunodai in addition to 12 isolates of an unidentified Eimeria species. Efficacy of coccidial prophylactic feed additives (salinomycin and diclazuril) and coccidial water medicaments (amprolium, ethopabate and toltrazuril) were studied in Japanese quails experimentally infected at fourteenth days old with 4.1×104 E. tsunodai sporulated oocysts. All groups which inoculated with E. tsunodai and received any one of the tested drugs had better clinical signs, mortality rate, lesion scores, oocyst output, sporulation percent, weight gain, feed consumption and FCR than the infected non treated group. Although all tested anticoccidials significantly improved the adverse effects of coccidia, yet there was no absolute superiority of any tested drug than the others. Generally we can say that water medicaments gave much better results in E. tsunodai infected quails than the feed additive anticoccidials.
https://ejvs.journals.ekb.eg/article_3591_9c43a9c2b7f90e606bc0a82acb6d1c63.pdf
Coccidia
Eimeria tsunodai
Quil
salinomycin
Treatment
eng
National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)
Egyptian Journal of Veterinary Sciences
1110-0222
2357-089X
2016-12-01
47
2
117
131
10.21608/ejvs.2016.1098
1098
Original Article
Pathogenesis of Experimental Salmonella Gallinarum Infection (fowl typhoid) in Broiler Chicks
Hamdy Soufy
hamdysoufy@yahoo.com
1
Mahmoud Gab-Allah
mahmoud.gaballah@fvtm.bu.edu.eg
2
Ahmed Tantawy
ahmadtantawy2007@yahoo.com
3
Ahmed Fotouh
sumerahmed2001@yahoo.com
4
Soad Nasr
soadnasr@yahoo.com
5
Department of Parasitology and Animal Diseases, National Research Centre, 33 Bohouth Street, Dokki, Post Box 12622, Giza, Egypt.
Department of Pathology, Faculty of Veterinary Medicine, Benha University, 13736 Moshtohour, Toukh, Qalioubeya, Egypt.
Department of Pathology, Faculty of Veterinary Medicine, Benha University, 13736 Moshtohour, Toukh, Qalioubeya, Egypt.
Department of Parasitology and Animal Diseases, National Research Centre, 33 Bohouth Street, Dokki, Post Box 12622, Giza, Egypt.
Department of Parasitology and Animal Diseases, National Research Centre, 33 Bohouth Street, Dokki, Post Box 12622, Giza, Egypt.
THIS study aimed to investigate the pathogenesis of locally isolated Salmonella Gallinarum biovar Gallinarum in experimentally infected Broiler chicks. One hundred and forty Broiler chicks -one day old- were randomly divided into two groups. The first group was kept as a control group. The second group was inoculated with S. Gallinarum biovar Gallinarum at a dose 0.2ml of sterile saline containing 3×108 CFU/ml through crop gavage. After inoculation, all experimental birds were kept under strict daily observation for recording clinical signs and mortality rate. Tissue samples were collected from dead and sacrificed chicks of each group at the 12th h, 1st, 2nd, 3rd, 4th, 7th, 14th, 21st and the 28th day post infection (dpi). Postmortem examination revealed severe congestion of internal organs. Microscopical findings illustrated congestion, thrombosis, focal hemorrhages, aggregation of bluish masses of bacterial colonies scattered in the hepatic parenchyma and thickening of hepatic capsule with bile duct hyperplasia. Fibrinous and granulomatous pericarditis were seen. Pulmonary congestion and thrombosis with focal hemorrhages and enteritis with desquamation of villar epithelium and goblet cells activation were observed. In conclusion, experimental inoculation of chicks with S. Gallinarum biovar Gallinarum was characterized by early septicemic lesions followed by perihepatitis, serofibrinous pericarditis and enteritis appeared after the 2nd dpi.
https://ejvs.journals.ekb.eg/article_1098_02837ae815febf27b9b714103ad777d2.pdf
Fowl typhoid
Salmonella Gallinarum
pathogenesis
Pathology
eng
National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)
Egyptian Journal of Veterinary Sciences
1110-0222
2357-089X
2016-12-01
47
2
133
149
10.21608/ejvs.2017.398.1008
2613
Original Article
Molecular Characterization of Infectious Bronchitis Virus in Chicken
heba el behery
hebanabilhassan@yahoo.com
1
sanaa awad
salama_sanaa@yahoo.com
2
samy kasem
samy_kasem1976@yahoo.com
3
poultry diseases
Professor of poultry and Rabbit Diseases, Faculty of Veterinary Medicine, Mansoura University
Associate professor of Virology, Faculty of Veterinary Medicine, Kafrelsheikh University
INFECTIOUS BRONCHITIS (IB) is a highly contagious ………disease of serious economic importance in the poultry industry worldwide. Diagnosis of Infectious Bronchitis Virus (IBV) infections in Egypt during 2013 - 2016 among poultry flocks. A total of 41 chicken flocks were examined (40 broiler flock and one breeder flock). Results revealed that 21 chicken flocks were found positive IBV by N gene conventional RT-PCR and real time RT-PCR. The samples were isolated and propagated in commercial embryonated chicken eggs (ECE) with result revealed that only one sample that showed curling and dwarfing of the embryo after the second passage while 10 samples were HA positive that suggest mixed infection of IBV with other viruses such as Newcastle Virus or Avian Influenza Virus. Furthermore, the allantoic fluids of the positive samples were subjected to specific S1 PCR to differentiate strains of IBV. The absence of positive reaction of S1gene conventional RT-PCR may confirm the involvements of other respiratory pathogens in chickens as Newcastle disease, avian influenza and mycoplasmosis that masked IBV infections. In conclusion, our results confirmed the detection of IBV in chicken flocks in Egypt which responsible for the respiratory problems in poultry farms and diagnosis with Real-time PCR because it is more rapid, sensitive, and reproducible, and the risk of carryover contamination is minimized compared to conventional PCR.
https://ejvs.journals.ekb.eg/article_2613_0cc157378f3265b26b59d0ca150a75dc.pdf
IBV
Diagnosis
RT-PCR
Egypt
eng
National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)
Egyptian Journal of Veterinary Sciences
1110-0222
2357-089X
2016-12-01
47
2
151
164
10.21608/ejvs.2016.3585
3585
Original Article
Zoonotic Importance of Salmonellosis in Chickens and Humans at Qualyobia Province
Lobna Salem
ellaithys@yahoo.com
1
Maysa A. I. Awadallah
maysavet@hotmail.com
2
nashwa O.K.
nashwakhalifa5@gmail.com
3
Marwa salem
marwavet91@yahoo.com
4
professor and head of zoonoses department, Fac. Vet. Med. Benha Univ
Department of Zoonoses, Fac. Vet. Med. Zagazig Univ.
Department of Zoonoses, Fac. Vet. Med. Benha Univ
department of zoonoses, Faculty of veterinary medecine. Benha university
THIS study aimed to isolate and identify Salmonella spp. From human and chickens by using culture method, serotyping, antimicrobial sensitivity test and detection of virulence genes (invA, avrA, bcfC, stn). A total of 245 samples and swabs including (36 cloacal swabs, 17 intestinal contents, 60 chicken product[ luncheon, nuggets , kofta, pane, 15 of each], 45 chicken meat[ breast, thigh, wing, 15 of each], 20 hand swabs , 27 stool (diarrheic and non- diarrheic ) and 40 serum) from humans for Widal test. The results revealed that 31 samples and swabs were positive to Salmonella, 6 isolates were isolated from broilers cloacal swabs, 7 isolates were isolated from chicken meat (4 in thigh samples and 3 in breast samples and swabs), 2 isolates were isolated from chicken products (one isolate in kofta sample and one isolates in pane swabs), 3 isolates were isolated from intestinal swabs, 4 isolates were isolated from hand swabs from workers in farms and poultry shops and 4 isolates were isolated from stool of diarrheic and non-diarrheic persons while 5 isolates were isolated from serum. Serotyping revealed 9 strains of S. Gallinarumand S. subspp. Salamae mainly in cloacal swabs, while S. Typhimurium, S. Enteritidis, S. Kentucky, S.Tsevie, S. Colindale, S. Papauna and S. Lagoswere isolated from chicken meat, its products, hand swab, intestinal swabs and stool. S. Typhi and Paratyphi A, B were isolated from serum of patients by Widal test. Nine strains were tested against 6 commercial antibiotics and revealed that all strains were sensitive to levofloxacin and amikacin while were resistant to erythromycin by 100% and ampicillin, tetracycline and cefexime shown viability in sensitivity. Widal test revealed S. Typhi and Paratyphi A, B by a titer of 1:320 for O, H antigens in S. Typhi, Paratyphi A and B. The study detected inv A, avr A, bcf C, stn genes in 11 S.Enteritidis and 3 S. Typhimurium that isolated from human and poultry and RAPD PCR revealed relationships in S. Enteritidis that isolated from human and poultry.
https://ejvs.journals.ekb.eg/article_3585_1e8f5ee7034e7a95f8b3961509a10641.pdf
Salmonellosis
chickens
humans
Zoonotic importance