Document Type : Original Article
Authors
1
Department of Microbiology, College of Veterinary Medicine, University of Baghdad, Baghdad, Iraq
2
Department of Internal and Preventive Veterinary Medicine, College of Veterinary Medicine, University of Baghdad, Baghdad, Iraq
Abstract
This Study aimed to comparison between diagnostic methods to evaluated the prevalence of Vibrio cholerae .A total of 90 samples include 40 birds faeces were collected from bird farms and birds breeding stores for Ducks, chickens, Pigeons, Pet birds and 50 hand swab from owners of birds (Males and Females) .collection from different places Al-hurya city, Abu Ghraib city, AL-Yarmouk area ,University district area in Baghdad Province, at the period of February 2021 to February 2022, in order to evaluate the routine laboratory diagnostic procedures in the diagnosis of V. cholerae isolates and compared them with molecular methods as Polymerase chain reaction (PCR).V.cholerae has been isolated and identified by using culturing method in addition to biochemical tests, Vitek2 system. The results showed that in culturing method percentage of isolation (43.33%)from birds feces, and (33.33%) in hand swabs,in biochemical tests(33.33%) in birds feces, and (10%)from hand swabs in Vitek2 system (6.7%)from each birds feces and hand swabs. PCR technique was used to detect sodB Gene encoding to outer membrane protein of V. cholerae ,on the PCR results, the rate of V. cholerae isolation from birds feces samples was 23.3 %. and 6.66% from hand swab samples,in Comparison PCR results showed that there were high sensitivity 100% with cultural, biochemical test , while 44% with Vitek2 system respectively.in presnt study found that diagnosis of V.cholerae by routine diagnostic may be misidentifications and using of PCR more faster, accurate ,sensitive and specific for correct identification.
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